Szabó, Nikoletta and Ajduković, Jovana and Djurendić, Evgenija and Sakač, Marija and Ignáth, Imre and Gardi, János and Mahmoud, Gábor and Klisurić, Olivera and Jovanović-Šanta, Suzana and Penov Gaši, Katarina and Szécsi, Mihály (2015) Determination of 17α-hydroxylase-C<sub>17,20</sub>-lyase (P450<sub>17α</sub>) enzyme activities and their inhibition by selected steroidal picolyl and picolinylidene compounds. Acta Biologica Hungarica, 66 (1). pp. 41-51. ISSN 0236-5383
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Abstract
17α-hydroxylase-C<sub>17,20</sub>-lyase (P450<sub>17α</sub>) is a key regulator enzyme of the steroid hormone biosynthesis in both the adrenals and the testes. Inhibition of this enzyme can block androgen synthesis in an early step, and may thereby be useful in the treatment of several androgen-dependent diseases. We developed radio-substrate in vitro incubation methods for the determination of the distinct 17α-hydroxylase and C<sub>17,20</sub>-lyase activities of the enzyme using rat testicular homogenate as enzyme source. With this method we have studied the inhibiting activity of selected steroidal picolyl and picolinylidene compounds. Tests revealed a substantial inhibitory action of the 17-picolinyliden-androst-4-en-3-one compound.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
| Depositing User: | Ágnes Sallai |
| Date Deposited: | 30 Jun 2016 08:41 |
| Last Modified: | 31 Mar 2017 23:15 |
| URI: | http://real.mtak.hu/id/eprint/36845 |
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