Kim, Yoon J. and Lim, Mi S. and Kim, Su M. and Ryu, Ki H. and Choi, Sun H. (2015) Molecular characterization of the Tobacco rattle virus RNA2 genome isolated from Gladiolus. Acta Biologica Hungarica, 66 (2). pp. 222-230. ISSN 0236-5383
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Abstract
Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
| Depositing User: | Ágnes Sallai |
| Date Deposited: | 30 Jun 2016 08:58 |
| Last Modified: | 30 Jun 2017 23:18 |
| URI: | http://real.mtak.hu/id/eprint/36866 |
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