Stefan, Andrei and Rosu, Craita M. and Stedel, Catalina and Gorgan, Lucian D. and Efrose, Rodica C. (2015) RAPD-inferred genetic variability of some indigenous Rhizobium leguminosarum isolates from red clover (Trifolium pratense L.) nodules. Acta Biologica Hungarica, 66 (3). pp. 316-325. ISSN 0236-5383
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Abstract
The application of commercial rhizobial inoculants to legume crops is proving to be an alternative to synthetic fertilizer use. The challenge for sustainable agriculture resides in the compatibility between crop, inoculants and environmental conditions. The evaluation of symbiotic efficiency and genetic diversity of indigenous rhizobial strains could lead to the development of better inoculants and increased crop production. The genetic variability of 32 wild indigenous rhizobial isolates was assessed by RAPD (Random Amplified Polymorphic DNA). The strains were isolated from red clover (Trifolium pratense L.) nodules from two distinct geographical regions of Northern and Eastern Romania. Three decamer primers were used to resolve the phylogenetic relationships between the investigated isolates. Cluster analysis revealed a high diversity; most strains clustered together based on their geographical location.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
Depositing User: | Ágnes Sallai |
Date Deposited: | 30 Jun 2016 09:03 |
Last Modified: | 30 Sep 2017 23:15 |
URI: | http://real.mtak.hu/id/eprint/36875 |
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