Virulence determination of Beauveria bassiana isolates on a predatory hemipteran, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)

Firouzbakht, H. and Zibaee, A. and Hoda, H. and Sohani, M. M. (2015) Virulence determination of Beauveria bassiana isolates on a predatory hemipteran, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae). Acta Phytopathologica et Entomologica Hungarica, 50 (1). pp. 115-125. ISSN 0238-1249

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Abstract

Virulence of the two isolates of Beauveria bassiana, BB2 and AM-118, were evaluated on adults of a predatory hemipteran, Andrallus spinidens Fabricius by conidial bioassay and enzymatic activities. Results of the bioassay revealed LC₅₀ of 37×10⁴ and 15×10³ spore/ml for isolates BB₂ and AM-118, respectively. Activities of chitinase, lipase and ALP showed the higher activity in the media inoculated by AM-118 while no statistical differences were observed in activity of ACP. Although no statistical differences were found in general protease and Pr₁ activities but activity of Pr₂ in AM-118 was significantly higher than that of BB₂. Activity of general esterases demonstrated no statistical differences when α- and β-naphtyl acetate were used as substrates but activity of glutathione S-transferase in AM-118 was higher than that of BB2 by using CDNB and DCNB as specific reagents. Results of the current study indicated higher virulence of isolate AM-118 against adults of A. spinidens by lower LC₅₀ value and higher activities of the enzymes involved in pathogenicity. Recruiting of these isolates against C. suppressalis must be considered by their adaptability of A. spinidens. Moreover, AM-118 has been isolated from rice fields of northern Iran, so it may somehow indicate a type of host-microorganism interaction.

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