Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect

Lopatina, Ekaterina V. and Kipenko, AV and Penniyaynen, VA and Pasatetskaya, NA and Djuric, D and Krylov, BV (2015) Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect. Acta Physiologica Hungarica, 102 (2). pp. 137-142. ISSN 0231-424X

Preview

Text 036.102.2015.2.4.pdf Download (313kB) | Preview

Abstract

Homocysteine thiolactone was demonstrated to inhibit the growth of 10–12-day-old chicken embryo cardiac tissue explants at 7 × 10⁻⁹ −1 × 10⁻³ M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10⁻³ M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10⁻³ M) and ouabain at concentrations regulating the signal-transducing (1 × 10⁻¹⁰ M) and pumping (1 × 10−8 M) functions of Na⁺,K⁺ -ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na⁺,K⁺ -ATPase pumping function.

Actions (login required)

Edit Item