Hydroponic culturing upregulates sucrose and glutamine metabolism by enhancing their utilization via intermediates of aerobic pathway in wheat

Kaur, B. and Asthir, B. (2016) Hydroponic culturing upregulates sucrose and glutamine metabolism by enhancing their utilization via intermediates of aerobic pathway in wheat. Cereal Research Communications, 44 (3). pp. 381-392. ISSN 0133-3720

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Abstract

Two wheat genotypes were grown in hydroponic culture, containing 4 mM KNO₃, NH₄Cl and NH₄NO₃. Activities of N metabolizing enzymes, aminotransferases, carbohydrate and TCA cycle enzymes were analyzed along with protein, amino acid, N, sugar content and growth parameters in shoot and root. After 12 days, the size of shoot and root system decreased significantly when plants were supplied with NH₄Cl as exclusive N source. Under NH₄NO₃ growth parameters, N and carbon metabolism were elevated as compared to NH₄Cl but less than KNO₃ source indicating inhibition of NH₄⁺ toxicity by NO₃⁻ uptake. Our results suggested that GDH, aminotransferases and PEPC play an important role in ammonium detoxification by its incorporation into amino acids. Thus, the morphologic differences among plants growing in NH₄⁺ or NO₃⁻ nutrition confirm the hypothesis that N source determines the growth habit of plant in wheat by modulating the endogenous levels of protein and sugar content.

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