Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

Aekbote Lakshman Rao, Badri and Fekete, Tamás and Jacak, Jaroslaw and Vizsnyiczai, Gaszton and Ormos, Pál and Kelemen, Lóránd (2016) Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells. BIOMEDICAL OPTICS EXPRESS, 7 (1). pp. 45-56. ISSN 2156-7085

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Abstract

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation. (C)2015 Optical Society of America

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