Study of biofilm formation by total-reflection X-ray fluorescence spectrometry

Kröpfl, K. and Záray, Gyula and Vladár, P. and Mages, M. and Ács, Éva (2003) Study of biofilm formation by total-reflection X-ray fluorescence spectrometry. Microchemical Journal, 75 (2). pp. 133-137. ISSN 0026-265X

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Abstract

A total reflection X-ray fluorescence spectrometric method was developed for elemental analysis of natural biofilms grown on polycarbonate substrates in the Lake Velence. For the duration of 9 weeks long growing period, two substrates were removed weekly from the lake and investigated by analytical and algological methods. The total biomass production achieved its highest value after 7 weeks. Ca, Sr and Ti, as well as Fe, Mn, K and Zn showed their maximum concentrations in the biofilms after 5 and 6-7 weeks, respectively. The enrichment factors of the 6 weeks old biofilm for the detected seven elements amounted to 10(3)-10(4). The recommended colonization time for biomonitoring of the Lake Velence is 6 weeks applying polycarbonate substrates.

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