Detection and Identification of Antibacterial and Antioxidant Components of Essential Oils by TLC-Biodetection and GC-MS

Móricz, Ágnes and Horváth, Györgyi and Böszörményi, Andrea and Ott, Péter (2016) Detection and Identification of Antibacterial and Antioxidant Components of Essential Oils by TLC-Biodetection and GC-MS. NATURAL PRODUCT COMMUNICATIONS, 11 (11). pp. 1705-1708. ISSN 1934-578X

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Abstract

Components of cinnamon bark, rosemary, clove and thyme essential oils were screened for antioxidant and antibacterial activity utilizing thin-layer chromatography (TLC) coupled with the DPPH• test and direct bioautography using Bacillus subtilis cells. The compounds in the active chromatographic zones were identified by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) after their elution. Seven antibacterial components were found: cinnamaldehyde and eugenol in cinnamon bark oil, 1,8-cineole, camphor, borneol and α-terpineol in rosemary oil, eugenol in clove oil and thymol in thyme oil. Only two of them, thymol and eugenol displayed a free radical scavenging effect.

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