Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

Sellyei, Boglárka and Ivanics, Éva and Magyar, Tibor (2013) Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene. Acta Veterinaria Hungarica, 61 (1). pp. 1-8. ISSN 0236-6290

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Abstract

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

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