Isolation and characterization of Xanthophyllomyces dendrorhous mutants with altered carotenoid content

Palágyi, Zs. and Linka, B. and Papp, T. and Vágvölgyi, Cs. (2006) Isolation and characterization of Xanthophyllomyces dendrorhous mutants with altered carotenoid content. Acta Alimentaria, 35 (2). pp. 223-228. ISSN 0139-3006

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Abstract

Cells of the astaxanthin-producing yeast Xanthophyllomyces dendrorhous were subjected to successive 60 Co and UV irradiation. Colonies exhibiting increased pigmentation were recovered from different non-selective plates. Mutant strains were subcultured to ensure their genetic homogeneity and their pigment production was characterized. Analysis of the metabolic patterns of 7 pigment-overproducing mutants (derived from 3 wild-type parental isolates) revealed different patterns of carotenoid production: the greatest increase in astaxanthin production (6.7-fold) was found for X. dendrorhous strain ATCC 24229/S119 (274 μ g g−1 dry weight). Mutant strains with increased total carotenoid content, but without significant change in astaxanthin production, were also isolated.

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