Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses

Kiss, I. and Germán, P. and Sámi, L. and Antal, Márta and Farkas, T. and Kardos, G. and Kecskeméti, S. and Dán, Á. and Belák, S. (2006) Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses. Acta Veterinaria Hungarica, 54 (4). pp. 525-533. ISSN 0236-6290

Text avet.54.2006.4.10.pdf Restricted to Repository staff only until 31 December 2026. Download (213kB)

Abstract

A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10-0.5 EID50/0.2 ml and 101.5 EID50/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs.

Actions (login required)

Edit Item