Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells. Short communication

Gálfi, P. and Neogrády, Susan and Gäbel, G. (2002) Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells. Short communication. Acta Veterinaria Hungarica, 50 (2). pp. 211-215. ISSN 0236-6290

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Abstract

Rate of amiloride-sensitive Na+ uptake into cultured rumen epithelial cells was studied in order to clarify the influence of culture conditions on Na+/H+ exchange (NHE). Cell cultures were exposed to Na-n-butyrate or not for seven days or subcultured. On the 14th day of culturing, primary cell cultures without butyrate exposure showed both non-stratified and stratified growth. Na-n-butyrate treated 14-day-old cultures and 3-day-old subcultures contained mostly non-stratified, i.e. non-keratinised cells. Both n-butyrate treatment and subculturing increased total and amiloride-sensitive Na+ uptake. Our results indicate that Na+ uptake via NHE is determined by the amount and the ratio of non-stratified (non-keratinised) cells.

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