The endosomal epsilon-coatomer protein is involved in human adenovirus type 5 internalisation

Jeney, Cs. and Banizs, Boglárka and Dobay, Orsolya and et al., (2002) The endosomal epsilon-coatomer protein is involved in human adenovirus type 5 internalisation. Acta Veterinaria Hungarica, 50 (4). pp. 481-489. ISSN 0236-6290

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Abstract

The effects of bafilomycin A1 and of the reduced level of endosomal epsilon-COP (coatomer protein) on the infectivity of human adenovirus type 5 were investigated in Coxsackie adenovirus receptor- (CAR-) transfected Chinese hamster ovary (CHO) cells. The endosomal proton pump inhibitor bafilomycin A1 was able to cause only partial inhibition. Using ldlF cells (an epsilon-COP thermosensitive mutant CHO cell line) the reduction of epsilon-COP level also had partial inhibitory effect. Based on these results and comparing them to existing models of the adenovirus entry, we propose a refined model in which there are two pathways of adenoviral entry: the first one involves the epsilon-COP as the downstream effector of the acidification and can be blocked by bafilomycin A1 and the second one is a pH-independent pathway.

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