Characterisation and effects of a xylanase enzyme preparation extracted from Thermomyces lanuginosus cultures

Kutasi, J. and Bata, Á. and Brydl, E. and Rafai, P. and Jurkovich, V. (2001) Characterisation and effects of a xylanase enzyme preparation extracted from Thermomyces lanuginosus cultures. Acta Veterinaria Hungarica, 49 (2). pp. 175-184. ISSN 0236-6290

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Abstract

This paper describes the production of an enzyme preparation from the fungus Thermomyces lanuginosus. Thermal resistance, pH stability and lignocellulolytic activity of the enzyme preparation high in xylanase were studied on a variety of grains and forages. The enzyme preparation preserved more than 70% of its original xylanase activity for 4 and 1 h at 60 and 70 °C, respectively. The xylanase activity remained over 80% when the preparation was incubated for 30 min at pH 4.5. In vitro digestibility studies indicated that the enzyme digested 7.5, 8.5 and 8.0% of the dry matter (DM) of barley meal, wheat bran and oat meal samples, respectively. When applying 60-min incubation, 7.5, 7.3 and 8.4% of DM of the oat straw, alfalfa hay and triticale straw was digested, respectively. When the time of digestion was increased to 360 min, the sunflower hull showed 15.8% DM digestibility.

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