Testing of a Chemiluminescence Enzyme Immunoassay for selective detection of E. coli O157 from ground beef samples

Domján Kovács, Hajnalka and Rásky, Klára (2001) Testing of a Chemiluminescence Enzyme Immunoassay for selective detection of E. coli O157 from ground beef samples. Acta Veterinaria Hungarica, 49 (4). pp. 377-383. ISSN 0236-6290

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Abstract

The aim of this study was to evaluate a Chemiluminescence Enzyme Immunoassay (CLIA) developed for the detection of E. coli O157:H7, using different E. coli O157 serotypes. The sensitivity and specificity of the kit were determined from the tenfold dilutions of the 24-hour broth cultures of the test strains. According to the results obtained in this trial, the sensitivity of the kit is 103–104 cells ml−1, and it is specific for E. coli O157. Twenty-five g ground raw beef samples were prepared and inoculated with E. coli O157:H7 at different CFU g−1. The samples were incubated in 225 ml of modified E. coli broth with novobiocin (mEC + n) at 42 °C for 4 h and the immunoassays were performed following the instructions of the manufacturer. According to the results obtained by the CLIA test 101–102 E. coli O157 g−1 can be detected from the sample. So this kit seems to be suitable for screening the samples before selective cultivation of E. coli O157:H7.

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