Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

Namula, Zhao and Kodama, Risa and Tanihara, Fuminori and Morita, Yasuhiro and Sato, Yoko and Wittayarat, Manita and Taniguchi, Masayasu and Otoi, Takeshige (2014) Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C. Acta Veterinaria Hungarica, 62 (1). pp. 106-116. ISSN 0236-6290

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Abstract

This study investigated the effects of skim-milk supplementation on the quality and penetrating ability of boar semen preserved at 15 °C. When boar semen samples were preserved in Modified Modena extender supplemented with various concentrations (0, 7.5, 15, 30 and 50 mg/mL) of skim milk powder at 15 °C for 4 weeks, higher sperm motility and viability were observed in the case of 7.5 mg/mL skim-milk supplementation compared with the control group (0 mg/mL) during the preservation (P < 0.05). When in vitro matured oocytes were co-incubated with boar sperm that had been preserved in Modified Modena extender with three different concentrations (0, 7.5 or 15 mg/mL) of skim milk powder at 15 °C for two weeks, there were no apparent effects of skim-milk supplementation on the rates of fertilisation and development to blastocysts of oocytes after co-incubation. However, the monospermic fertilisation rate of sperm preserved with 15 mg/mL skim milk powder was higher (P < 0.05) than that of fresh non-preserved sperm, but did not differ among the preservation groups. The results indicate that the supplementation of Modified Modena extender with 7.5 mg/mL skim milk powder improves the motility and viability, but not the penetrating ability, of sperm after liquid preservation for at least two weeks.

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