Dobrosavljević, Ivan and Vidanović, Dejan and Velhner, Maja and Miljković, Biljana and Lako, Branislav (2014) Simultaneous detection of vaccinal and field infectious bursal disease viruses in layer chickens challenged with a very virulent strain after vaccination. Acta Veterinaria Hungarica, 62 (2). pp. 264-273. ISSN 0236-6290
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Abstract
Infectious bursal disease virus is an important poultry pathogen. It is distributed worldwide and causes significant economic losses. In this study, a system was adopted for the simultaneous monitoring of vaccine and virulent strains using reverse transcription polymerase chain reaction (RT-PCR). After the decay of maternal antibodies, chickens were vaccinated at the age of 37 days with a virus of intermediate virulence and challenged at 5, 10 and 14 days post vaccination (dpv). The challenge was done with IBDV strain CH/99. Sequencing of the hypervariable region of VP2 has shown that CH/99 belongs to the very virulent group of viruses. The vaccine virus could be found in the bursa of Fabricius, spleen, thymus and bone marrow until 24 dpv. The CH/99 challenge virus was found in the bursa and lymphoid organs when chickens were challenged at 5 and 10 dpv. When challenge was performed at 14 dpv, the pathogenic virus could not be found in the bursa and other lymphoid organs.
Item Type: | Article |
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Subjects: | S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány |
Depositing User: | Erika Bilicsi |
Date Deposited: | 06 Mar 2017 13:34 |
Last Modified: | 04 Apr 2023 12:33 |
URI: | http://real.mtak.hu/id/eprint/49771 |
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