Szmolka, Ama and Szabó, Mónika and Kiss, János and Pászti, Judit and Adrián, Erzsébet and Olasz, Ferenc and Nagy, Béla (2017) Molecular epidemiology of the endemic multiresistance plasmid pSI54/04 of Salmonella Infantis in broiler and human population in Hungary. Food Microbiology. ISSN 0740-0020 (In Press)
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Abstract
Salmonella Infantis (SI) became endemic in Hungary where the PFGE cluster B, characterized by a large multiresistance (MDR) plasmid emerged among broilers leading to an increased occurrence in humans. We hypothesised that this plasmid (pSI54/04) assisted dissemination of SI. Indeed, Nal-Sul-Tet phenotypes carrying pSI54/04 occurred increasingly between 2011-2013 among SI isolates from broilers and humans. Characterization of pSI54/04 based on genome sequence data of the MDR strain SI54/04 indicated a size of ~277 kb and a high sequence similarity with the megaplasmid pESI of SI predominant in Israel. Molecular characterization of 78 representative broiler and human isolates detected the prototype plasmid pSI54/04 and its variants of together with novel plasmid associations within the emerging cluster B. To test in vitro and in vivo pathogenicity of pSI54/04 we produced plasmidic transconjugant of the plasmid-free pre-emergent strain SI69/94. This parental strain and its transconjugant have been tested on chicken embryo fibroblasts (CEFs) and in orally infected day old chicks. The uptake of pSI54/04 did not increase the pathogenicity of the strain SI69/94 in these systems. Thus, dissemination of SI in poultry could be assisted by antimicrobial resistance rather than by virulence modules of the endemic plasmid pSI54/04 in Hungary.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
Depositing User: | PhD Ama Szmolka |
Date Deposited: | 20 Mar 2017 15:56 |
Last Modified: | 04 Apr 2023 12:36 |
URI: | http://real.mtak.hu/id/eprint/50175 |
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