Prevalence of human herpesvirus 6A and 6B during pregnancy

Mészáros, Beáta and Sápy, Tamás and Gergely, Lajos and Csoma, Eszter (2014) Prevalence of human herpesvirus 6A and 6B during pregnancy. Acta Microbiologica et Immunologica Hungarica, 61 (3). pp. 309-316. ISSN 1217-8950

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Abstract

The aim of the present study was to assess the frequency of human herpesvirus 6A (HHV-6A) and human herpesvirus 6B (HHV-6B) infection during pregnancy. 100–100 blood samples were collected from pregnant and non-pregnant women, then nucleic acid was isolated from both plasma and leukocytes fraction. Nested and real-time PCR were used to detect and differentiate HHV-6A and HHV-6B DNA and to determine viral loads. Reverse transcription PCR (RT-PCR) for HHV-6 U79/80 mRNA was performed in order to reveal active HHV-6 replication.HHV-6A and HHV-6B active infections were not detected in blood samples neither from pregnant nor from non-pregnant women. Frequency of HHV-6B and HHV-6A latency did not show difference between the studied groups (15% vs. 16%). HHV-6B latency was dominant in both studied groups (14/15 and 15/16). Beside these results, in leukocyte samples of one pregnant and three non-pregnant women high HHV-6A viral loads (1.28 × 10⁵ − 5.07 × 10⁵ GEq / 1.5 × 10⁶ leukocytes) were detected, and viral DNA was also found in plasma samples. Although RT-PCR did not confirm virus replication, but chromosomal integration was also not proved unequivocally, the number of 0.08–0.33 HHV-6 copy / 1 leukocyte refers more to postnatal infection.

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