Isolation and characterization of the complete genome of barley yellow dwarf virus-GAV from wheat in Northwestern China

Song, S. and Tao, Y. and Zhao, L. and Liu, P. and Wu, Y. (2014) Isolation and characterization of the complete genome of barley yellow dwarf virus-GAV from wheat in Northwestern China. Cereal Research Communications, 42 (4). pp. 620-628. ISSN 0133-3720

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Abstract

Barley yellow dwarf virus-GAV (BYDV-GAV) is one of the most serious viruses on wheat in China. In this study, five BYDV-GAV isolates collected from five regions in Northwestern China were sequenced. The complete genome sequences generated in this study along with nine genome sequences of BYDV-GAV isolates available in GeneBank were compared and analyzed. The comparative analysis indicated that the complete genomes of BYDV-GAV showed a low level of genetic diversity with nucleotide sequence identities ranging between 97.0% and 99.7%, and the RNA-dependent RNA polymerase gene (ORF1 + ORF2) was the most variable within the complete genome. Phylogenetic analysis indicated that the BYDV-GAV isolates in Northwestern China could be divided into two groups. In addition, two potential recombination events were detected among the 14 BYDV-GAV isolates. This study provided a detailed description of molecular characterization of BYDV-GAV in Northwestern China based on the complete genome sequences, which increased the understanding of genetic diversity of barley yellow dwarf viruses.

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