Analysis of retrotransposition and DNA methylation in barley callus culture

Temel, Aslihan and Gozukirmizi, Nermin (2013) Analysis of retrotransposition and DNA methylation in barley callus culture. Acta Biologica Hungarica, 64 (1). pp. 86-95. ISSN 0236-5383

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Mature barley (Hordeum vulgare cv. Zafer-160) embryos were cultured on callus induction medium (MS+ 4 mg l<sup>−1</sup> Dicamba) for 30 days and embryogenic calli were transferred onto regeneration medium (MS+ 0.5 mg l<sup>−1</sup> trans-zeatin riboside). Callus induction percentage was 67.2%; embryogenic callus induction percentage was 42.3% and their regeneration percentage was 63.8%. Retrotransposon movements and methylation alterations in 15-day-old, 30-day-old calli and 4-day-old barley seedling (control) were investigated with Inter-Retrotransposon Amplified Polymorphism (IRAP) and Methylation-Sensitive Restriction Fingerprinting (MSRF), respectively. IRAP patterns were quite monomorphic however MSRF indicated increase in cytosine methylation during callus formation. Changes in retroelement movements and methylation alterations were evaluated and discussed in the light of literature.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Ágnes Sallai
Date Deposited: 20 Apr 2017 06:49
Last Modified: 20 Apr 2017 06:49

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