High frequency of unusual high molecular weight glutenin alleles in 232 tetraploid durum wheat accessions (Triticum turgidum L. ssp. durum Desf)

Elfatih, S. and Peng, Y. and Ma, J. and Peng, J. and Sun, D. and Ma, W. (2013) High frequency of unusual high molecular weight glutenin alleles in 232 tetraploid durum wheat accessions (Triticum turgidum L. ssp. durum Desf). Cereal Research Communications, 41 (4). pp. 583-592. ISSN 0133-3720

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Abstract

A total of 232 accessions of tetraploid species, durum wheat (Triticum turgidum L. ssp. durum Desf., 2n=4x=28, AABB) with a widespread origin of various countries were used in this study. Their high molecular weight glutenin subunit (HMW-GS) composition was identified by Matrix-assisted laser desorption/ionization time-of-flight Mass Spectrometry (MALDI-TOF-MS). Among all accessions analyzed, 194 were homogeneous for HMW-GS, 38 were heterogeneous, and 62 possessed unusual or new subunits. The results revealed a total of 43 alleles, including 5 at Glu-A1 and 38 at Glu-B1, resulting in 60 different allele combinations. The Glu-B1 locus displayed higher variation compared with Glu-A1. Glu-A1c (55.2%) and Glu-B1aj (17.7%) were the most frequent alleles at Glu-A1 and Glu-B1, respectively. Two allele types (“null” and 1) at the Glu-A1 locus and three allele types (7OE + 8, 14+15, 8) at the Glu-B1 locus appeared to be the common types in the 232 accessions. A total of 23 new alleles represented by unusual subunits were detected at the Glu-A1 and the Glu-B1 locus.

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