Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels–Alder Reactions

Kozma, E. and Demeter, Orsolya and Kele, Péter (2017) Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels–Alder Reactions. CHEMBIOCHEM, 18. pp. 486-501. ISSN 1439-4227

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Abstract

Bio-ort hogona llabellin gschemes based on inverse-elec tron- deman dDiels–Ald er (IEDDA) cycloa ddition have attracted much attention in chem ical biology recently .The appeal ing features of this reactio n, such as the fast reactio nkinetics, fully bio-ort hogonal nature and high selectiv ity,have helped chem i- cal biologists gain deeper understandi ng of biochemic al pro- cesses at the molecular level.Listing the compo nents and dis- cussing the possib ilities andlimitations of thesereagent s, we provid earecent snapshot of the field of IEDDA -based biomo- lecular manipulatio nwith special focus on fluores cent modula- tion approaches throug hthe use of bio-orthogon alized build- ing blocks. At the end, we discuss challenges that need to be addres sed for further develop ments in order to overcome recent limita tions and to enabl eresearchers to answer biomo - lecular quest ions in more detail.

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