Cultivar identification by molecular markers

Bányai, J. and Szűcs, P. and Karsai, I. and Mészáros, K. and Kuti, Cs. and Láng, L. and Bedő, Z. (2006) Cultivar identification by molecular markers. Cereal Research Communications, 34 (2-3). pp. 865-870. ISSN 0133-3720

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Abstract

A total of 96 winter wheat ( Triticum aestivum L.) cultivars registered in Hungary were analysed using 15 wheat microsatellite markers located on different chromosome arms. Analyses revealed 91 SSR alleles with sizes ranging from 123–239 base pairs. The total number of alleles per locus ranged from 2 (Gwm664 and Gwm415) to 11 (Gwm219) with an average number of 6.1. The polymorphic information content (PIC) values ranged from 0.06 to 0.85 with an average number of 0.60 for all markers. Several markers included allele sizes characteristic of a single or a small number of cultivars. At most 9 SSR markers were required to distinguish the 96 cultivars, so the simple sequence repeats could serve as a relatively cheap, rapid method for identifying winter wheat cultivars.

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