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Expression of the 1D×5 high molecular weight glutenin subunit protein in transgenic rice

Oszvald, M. and Jenes, B. and Tömösközi, S. and Békés, F. and Tamás, L. (2007) Expression of the 1D×5 high molecular weight glutenin subunit protein in transgenic rice. Cereal Research Communications, 35 (4). pp. 1543-1549. ISSN 0133-3720

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Abstract

We generated and analysed transgenic rice lines synthesizing substantial amounts of high-molecular-weight glutenin subunit (HMW-GS) from wheat. Particle bombardment has been used to transform rice cultivars ( Orysa sativa L.) with a cassette carrying the gene of 1D×5 HMW glutenin subunit. Twelve independent lines were recovered and PCR results on genomic DNS confirmed the integration of the transgene into it. Five lines set seeds. Seeds were analysed by SDS-PAGE and Western blot and we proved the presence of foreign protein in the starchy endosperm. The amount of 1D×5 HMW-GS in rice endosperm represents 0.75–3.18% of the alcohol soluble proteins. These results are the first example of significantly changing storage protein composition of rice exploiting the method of gene technology. This alteration may have considerable effect on the functional properties, including strength and stability of the dough made of transgenic rice flour.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: Barbara Payer
Date Deposited: 30 Nov 2017 19:06
Last Modified: 30 Nov 2017 19:06
URI: http://real.mtak.hu/id/eprint/59359

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