Characterization of HMW-GS genes Dx5 ^t and Dy12 ^t from Aegilops tauschii accession with subunit combination Dx5 ^t + Dy12 ^t

Yan, Z. and Guo, Z. and Liu, D. and Dai, S. and Wei, Y. and Zheng, Y. (2008) Characterization of HMW-GS genes Dx5 ^t and Dy12 ^t from Aegilops tauschii accession with subunit combination Dx5 ^t + Dy12 ^t. Cereal Research Communications, 36 (3). pp. 477-487. ISSN 0133-3720

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Abstract

Aegilops tauschii is the generally accepted D genome diploid donor of hexaploid wheat. The significance of Ae. tauschii HMW-GS genes on bread-making properties of bread wheat has been well documented. Among them, Ae. tauschii HMW-GS Dx5 ^t +Dy12 ^t was thought as the pair with potentially value in endowing synthetic hexaploid wheat with good end-use qualities. In this paper, we isolated and sequenced genes Dx5 ^t and Dy12 ^t from Ae. tauschii accession As63. Amino acid sequence comparison indicated that Dy12 ^t from Ae. tauschii is more similar to Dy10 rather than Dy12 of bread wheat. The sequence of Dx5 ^t in Ae. tauschii accession As63 showed higher similarity to that of Dx5 in bread wheat than others. However, it is notable that Dx5 ^t lacked the additional cysteine residue in Dx5, which is responsible for good bread-making quality in common wheat. Moreover, compared to Dx5, Dx5 ^t has an extra hexpeptide repetitive motif unit (SGQGQQ) as well as five amino acid substitutions.

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