Comparison of PCR-based DNA markers for using different Lr19 and Lr24 leaf rust resistance wheat sources

Uhrin, A. and Láng, L. and Bedő, Z. (2008) Comparison of PCR-based DNA markers for using different Lr19 and Lr24 leaf rust resistance wheat sources. Cereal Research Communications, 36 (4). pp. 533-541. ISSN 0133-3720

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Abstract

Both resistance genes Lr19 and Lr24 originate from Agropyron elongatum . The gene Lr24 is derived from two different translocations: 1BS/3Ag (‘Amigo’) or 3DS/3Ag (‘Agent’). The use of molecular markers makes selection easier during the breeding process as well as in the selection of the parents. In this study, two markers were used to identify the gene Lr19 (GbF/R ₁₃₀ , SCS265 ₅₁₂ ) and four different markers (J9/1-2 ₃₁₀ , SC-H5 ₇₀₀ , SCS1302 ₆₁₃ and SCS1326 ₆₀₇ ) were available to search for the gene Lr24 . The GbF/ _R130 marker for gene Lr19 worked well, but the SCAR marker SCS265 ₅₁₂ proved to be easier to use in MAS. SCAR markers SCS1302 ₆₁₃ and SCS1326 ₆₀₇ proved to be highly reliable and effective for gene Lr24 not only in Agent-derived sources but also in ‘Amigo’ derivatives. The STS marker J9/1-2 ₃₁₀ and the SCAR marker SC-H5 ₇₀₀ required several modifications and were effective only in ‘Agent’ offsprings.

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