Identification of novel ω -gliadin gene in Aegilops tauschii using RFLP

Hassani, M. and Naghavi, M. and Shariflou, M. and Sharp, P. (2009) Identification of novel ω -gliadin gene in Aegilops tauschii using RFLP. Cereal Research Communications, 37 (1). pp. 75-82. ISSN 0133-3720

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Abstract

A RFLP approach was used to investigate polymorphism of ω -gliadin genes in Ae. tauschii using a F ₂ population from the cross of accessions AUS18913 and CPI110856. A set of 150 F ₂ progenies was genotyped by acid polyacrylamide gel electrophoresis (A-PAGE) and only one recombinant line of Gli-D ^t 1/Gli-D ^t T1 was observed. Twelve restriction enzymes were initially tested on genomic DNA of the two parents of which four restriction enzymes revealed polymorphism. Of these four, only Dra I was associated with the novel ω -gliadin gene (T ₁ ) using a 1,200 bp DNA fragment of a ω -gliadin gene as a gene-specific probe. The ω -gliadin gene (T ₁ ) may be of interest for further studies relating storage proteins and wheat bread-making quality.

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