Development of a molecular marker for simultaneous selection of Rph7 gene and effective Mla alleles in barley

Sedláček, T. and Stemberková, L. (2010) Development of a molecular marker for simultaneous selection of Rph7 gene and effective Mla alleles in barley. Cereal Research Communications, 38 (2). pp. 175-183. ISSN 0133-3720

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Abstract

Barley ( Hordeum vulgare L.) is one of the most economically important crops around the world. Diseases caused by fungi and viruses significantly reduce yield. The most important fungal diseases are leaf rust caused by Puccinia hordei , and powdery mildew caused by Blumeria graminis f. sp. hordei . The most economical and ecologically friendly way to avoid losses caused by these diseases is growing resistant varieties. Concerning practical breeding for powdery mildew and leaf rust resistance, simple, cheap and robust selection methods are required. Marker assisted selection (MAS) is of great potential to fulfil this demand. In this paper, we present development of a robust duplex marker for simultaneous selection of Rph7 , and presence of one of the following Mla alleles: Mla16, Mla19, Mla20, Mla21, Mla27 or Mla28 .

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