Characterization of wheat yellow rust resistance gene Yr17 using EST-SSR and rice syntenic region

Jia, J. and Li, G. and Liu, C. and Lei, M. and Yang, Z. (2011) Characterization of wheat yellow rust resistance gene Yr17 using EST-SSR and rice syntenic region. Cereal Research Communications, 39 (1). pp. 88-99. ISSN 0133-3720

Text crc.39.2011.1.9.pdf Restricted to Repository staff only until 31 March 2031. Download (574kB)

Abstract

Wheat yellow rust resistance gene Yr17 was originated from the wheat-Aegilops ventricosa introgression, and still effective on the adult plant in Southern China. The previous studies located the gene Yr17 on the translocation of 2NS-2AS using the molecular and cytological markers. In the present study, we screened new PCR-based markers to map the gene Yr17 region from the investigation of a segregating 120 F2 population. All markers including four EST-PCR markers, a SCAR (sequence characterized amplified region) and a PLUG (PCR based landmark unique gene) marker specific to Yr17 gene were mapped on the chromosome 2AS, and located on the chromosomal deletion bin 2AS5-0.8–1.00 region. Based on the wheat-rice collinearity, we found that the sequences of the Yr17 gene linked markers were comparatively matched at rice chromosome 4 and chromosome 7. However, the identified closely linked genomic sequence of Yr17 gene is most likely collinear with genomic region of rice chromosome 4. The newly produced PCR based markers closely linked to Yr17 gene will be useful for the marker-assisted selection in wheat breeding for rust resistance.

Actions (login required)

Edit Item