Genetic diversity of gluten proteins in T. turgidum L.

Riefolo, C. and Ficco, D. and Cattivelli, L. and Vita, P. (2011) Genetic diversity of gluten proteins in T. turgidum L. Cereal Research Communications, 39 (3). pp. 405-414. ISSN 0133-3720

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Abstract

The genetic variations of high and low molecular weight glutenin subunits (HMW-GS and LMW-GS) as well as of ω- and γ-gliadins in 562 accessions of 7 tetraploid Triticum turgidum L. subspecies were investigated using sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE). A total of 26 HMW-GS alleles (7 at Glu-A1 and 19 at Glu-B1 loci) with 63 allelic combinations, as well as 11 LMW-GS alleles (5 at Glu-A3, 4 at Glu-B3 and 2 at Glu-B2 loci) with 26 allelic combinations, were detected. Two novel HMW-GS, called B1cf and B1cg, were discovered in T. dicoccum, B1cg was also found in T. turanicum. The Glu-B1 locus showed the highest values of genetic diversity index (H), with a mean of 0.72. As regards gliadins, 8 alleles at Gli-B1 locus have been found. The dendrogram based on allelic frequencies, revealed that T. durum, T. carthlicum and T. polonicum grouped a part from the other subspecies. This behaviour suggested probably different evolutive pathways among the tetraploid wheats.

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