Screening adenoviruses in stool samples: evaluation of a genus-specific monoclonal antibody based enzyme immunoassay

Bányai, K. and Máté, Zsanett and Ádám, Éva and Új, Mária and Nász, I. and Szűcs, G. (2003) Screening adenoviruses in stool samples: evaluation of a genus-specific monoclonal antibody based enzyme immunoassay. Acta Microbiologica et Immunologica Hungarica, 50 (1). pp. 23-32. ISSN 1217-8950

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Abstract

To screen fecal samples for adenovirus antigens a genus-specific monoclonal antibody based enzyme immunoassay was developed. In a comparative analysis with commercial latex agglutination test, high sensitivity was demonstrated. The assay did not detect other viruses usually found in faeces suggesting its specificity. One hundred and eighty stool samples collected in Baranya County were tested and 13 (7.2%) of them showed reactivity. The application of our immunoassay combined with other, more sophisticated methods may help us to determine the serotype specificity of these adenovirus isolates and assess the importance of adenoviruses in viral gastroenteritis.

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