Heme-induced contractile dysfunction in Human cardiomyocytes caused by oxidant damage to thick filament proteins

Gerardo, Alvarado and Jeney, Viktória and Tóth, Attila and Csősz, Éva and Kalló, Gergő and Huynh, An T. and Hajnal, Csaba and Kalász, Judit and Pásztor, Enikő T. and Édes, István and Gram, Magnus and Akerström, Bo and Smith, Ann and Eaton, John W. and Balla, György and Papp, Zoltán and Balla, József (2015) Heme-induced contractile dysfunction in Human cardiomyocytes caused by oxidant damage to thick filament proteins. FREE RADICAL BIOLOGY AND MEDICINE. ISSN 0891-5849

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Abstract

Intracellular free heme predisposes to oxidant-mediated tissue damage. We hypothesized that free heme causes alterations in myocardial contractility via disturbed structure and/or regulation of the contractile proteins. Isometric force production and its Ca2+-sensitivity (pCa(50)) were monitored in permeabilized human ventricular cardiomyocytes. Heme exposure altered cardiomyocyte morphology and evoked robust decreases in Ca2+-activated maximal active force (F-o) while increasing Ca2+-independent passive force (F-passive). Heme treatments, either alone or in combination with H2O2, did not affect pCa(50). The increase in F-passive started at 3 mu M heme exposure and could be partially reversed by the antioxidant dithiothreitol. Protein sulthydryl (SH) groups of thick myofilament content decreased and sulfenic acid formation increased after treatment with heme. Partial restoration in the SH group content was observed in a protein running at 140 kDa after treatment with dithiothreitol, but not in other proteins, such as filamin C. myosin heavy chain, cardiac myosin binding protein C. and alpha-actinin. Importantly, binding of heme to hemopexin or alpha-l-microglobulin prevented its effects on cardiomyocyte contractility, suggesting an allosteric effect. In line with this, free heme directly bound to myosin light chain 1 in human cardiomyocytes. Our observations suggest that free heme modifies cardiac contractile proteins via posttranslational protein modifications and via binding to myosin light chain 1, leading to severe contractile dysfunction. This may contribute to systolic and diastolic cardiac dysfunctions in hemolytic diseases, heart failure, and myocardial ischemia-reperfusion injury. (C) 2015 Published by Elsevier Inc.

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