Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans

Erdei, Éva and Pusztahelyi, Tünde and Miskei, M. and Barna, Teréz and Pócsi, I. (2008) Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans. Acta Microbiologica et Immunologica Hungarica, 55 (3). pp. 351-361. ISSN 1217-8950

Text amicr.55.2008.3.6.pdf Restricted to Repository staff only until 30 September 2028. Download (132kB)

Abstract

Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli . Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns.

Actions (login required)

Edit Item