Screening and selection of lovastatin hyper-producing mutants of Aspergillus terreus using cyclic mutagenesis

Kaur, Harleen and Kaur, Amarjeet and Saini, H. S. and Chadha, B. S. (2009) Screening and selection of lovastatin hyper-producing mutants of Aspergillus terreus using cyclic mutagenesis. Acta Microbiologica et Immunologica Hungarica, 56 (2). pp. 169-180. ISSN 1217-8950

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Abstract

134 fungal cultures isolated from different soil samples were screened for lovastatin production. Of these, 38 isolates produced different levels of lovastatin. An Aspergillus terreus strain GD13 , producing 190 mg/l of lovastatin was selected and subjected to a rational mutation-selection programme based on the resistance to lovastatin and fatty acid synthase (FAS) inhibitors, viz., iodoacetamide and N-ethylmaleimide. After three cycles of mutagenesis, a hyper-producing mutant (EM19) exhibiting 7.5-fold (1424 mg/l) higher levels of lovastatin when compared to wild type parent strain was obtained.

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