Production and purification of low calcium response protein H of Chlamydophila pneumoniae

Faludi, Ildikó and Csanádi, Ágnes and Szabó, Ágnes Míra and Burián, Katalin and Endrész, Valéria and Miczák, A. (2009) Production and purification of low calcium response protein H of Chlamydophila pneumoniae. Acta Microbiologica et Immunologica Hungarica, 56 (4). pp. 389-397. ISSN 1217-8950

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Abstract

Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein.

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