Rapid identification of Iranian Acinetobacter Baumannii strains by single PCR assay using BLAoxa-51-like carbapenemase and evaluation of the antimicrobial resistance profiles of the isolates

Mahdi, Akbari and Mohammad, Niakan and Morovat, Taherikalani and Mhammad-Mahdi, Feizabadi and Namam-Ali, Azadi and Setareh, Soroush and Mohammad, Emaneini and Amir, Abdolkarimi and Abbas, Maleki and Ali, Hematian (2010) Rapid identification of Iranian Acinetobacter Baumannii strains by single PCR assay using BLAoxa-51-like carbapenemase and evaluation of the antimicrobial resistance profiles of the isolates. Acta Microbiologica et Immunologica Hungarica, 57 (2). pp. 87-94. ISSN 1217-8950

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Abstract

The rapid identification of relevant bacterial pathogens is of utmost importance in clinical settings. The aim of this study was to test a rapid identification technique for A. baumannii strains from Tehran Hospitals and to determine the antibiotic resistance profiles of the isolates. A hundred strains of Acinetobacter spp. grown from clinical specimens were identified as A. baumannii by conventional methods. Using PCR a blaOXA-51-like gene was detected in all A. baumannii isolates but not in other species of acinetobacter. More than half of the isolates proved resistant to a variety of antibiotics by the disk diffusion technique. The rate of resistance to gentamicin, imipenem, ampicillin-sulbactam and amikacin was determined to be 45%, 53%, 62% and 62%, respectively. Moreover, most isolates (more than 90%) showed resistance to cephalosporins. This study shows that the demonstration of the blaOXA-51-like gene is a reliable and rapid way for the presumptive identification of A. baumannii and reveals that the rate of antibiotic resistance is high in Iranian A. baumannii isolates to a variety of antibiotics.

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