Synergistic action of rutin and orthovanadate on nitric oxide release from mouse macrophage cells

Koncz, S. and Horváth, Edit J. (2000) Synergistic action of rutin and orthovanadate on nitric oxide release from mouse macrophage cells. Acta Physiologica Hungarica, 87 (1). pp. 53-66. ISSN 0231-424X

Preview

Text aphysiol.87.2000.1.6.pdf Download (287kB) | Preview

Abstract

The cooperative action of sodium orthovanadate (a putative protein-phosphotyrosine phosphatase inhibitor) and rutin (an effective superoxide scavenger) on the nitric oxide (NO) production of J774A.1 mouse macrophage cells has been investigated. Orthovanadate alone caused a mild but significant increase in NO production of the cells at its highest concentration used (500 μ M). Orthovanadate and rutin together caused a significant increase in the nitrite level of the supernatants of the J774A.1 cells after a 24-hour incubation period, in a concentration dependent manner. The optimal doses for orthovanadate and rutin were 50 μ M and 100 μ M, respectively. This cooperative action of rutin and orthovanadate was totally inhibitable by catalase, reduced glutathion, N-acetylcystein, cycloheximide, pyrrolidine dithiocarbamate (a putative NF- κ B inhibitor), genistein and tyrphostin-AG126 (two protein tyrosine-kinase inhibitors). Superoxide dismutase had no inhibitory effect. Orthovanadate and rutin (only together) could induce the oxidation of 2',7'-dichlorofluorescein-diacetate, a marker of hydrogen peroxide. This effect was inhibitable by reduced glutathion, a hydrogen peroxide specific scavenger. These findings suggest, that orthovanadate can induce the production of NO by J774A.1 macrophages not only by inhibition of protein tyrosine-phosphatases, but, using it with rutin, by increasing the level of hydrogen peroxide in the cells.

Actions (login required)

Edit Item