Inhibition of fungal plant pathogens by seed proteins of  Harpullia cupanioides (Roxb.)

Bharathimatha, C. and Doraiswamy, Sabitha and Velazhahan, R. (2002) Inhibition of fungal plant pathogens by seed proteins of  Harpullia cupanioides (Roxb.). Acta Phytopathologica et Entomologica Hungarica, 37 (1-3). pp. 75-82. ISSN 0238-1249

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Abstract

Seed extracts of 50 plant species belonging to different families were evaluated for their ability to inhibit growth of Trichoderma viride in vitro. Of the various seed extracts, the seed extracts of Harpullia cupanioides (Roxb) belonging to Sapindaceae family exhibited very high antifungal activity. The seed extract of H. cupanioides strongly inhibited the growth of Rhizoctonia solani, Curvularia lunata, Colletotrichum musae and Alter­naria alternata. Seed extract of H. cupanioides retained its antifungal acti­vity even after heating at 100 °C for 10 min or autoclaving at 121 °C for 20 min. For partial purification of antifungal proteins, H. cupanioides seed extract was subjected to ammonium sulphate fractionation followed by gel filtration on Sephadex G-200 column. The fractions from sephadex G-200 were individually tested for their antifungal activity against T. viride. SDS-PAGE analysis of the fractions from Sephadex-G200 column indi­cat­ed that the fractions with antifungal activity contained a 68-kDa band as well as other low molecular weight protein bands. The N-terminal amino acid sequence of the 68-kDa protein (13 residues) was determined by Edman degradation. However, comparison with sequences in the GenBank database (Swiss Prot) did not reveal any homology with known protein sequences.

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