Additional polymorphic microsatellite loci for detailed population genetic studies of Lethrus apterus (Coleoptera: Geotrupidae)

Rácz, Rita and Bereczki, Judit and Kosztolányi, András and Horváth, Attila and Sziráki, Szabolcs (2017) Additional polymorphic microsatellite loci for detailed population genetic studies of Lethrus apterus (Coleoptera: Geotrupidae). ACTA ZOOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE, 63 (3). pp. 355-360. ISSN 1217-8837

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Abstract

In this study we attempted to isolate 13 additional microsatellites from the beetle Lethrus apterus and then to use them to screen for polymorphisms in 35 specimens collected from the northern Hungarian mountains. The newly developed markers presented in this paper are polymorphic, including between two and seven detected alleles per locus. The observed and expected heterozygosity per locus ranged between 0.057 and 0.686 and between 0.056 and 0.756, respectively, while two loci showed significant deviations from Hardy-Weinberg equilibrium. These deviations can be explained by the presence of null alleles. All loci cross- amplified in at least two out of four congeneric Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun). The polymorphic microsatellite markers we present for L. apterus will be valuable for future population genetic studies.

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