Oxidative stress in the rats brain capillaries in sepsis - the influence of 7-nitroindazole

Ninković, M. and Maličević, Z. and Jelenković, A. and Jovanović, D.M. and Đukić, M. and Vasiljević, I. (2006) Oxidative stress in the rats brain capillaries in sepsis - the influence of 7-nitroindazole. Acta Physiologica Hungarica, 93 (4). pp. 315-323. ISSN 0231-424X

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Abstract

As a part of blood-brain barrier, brain capillaries participate in pathophysiological events during systemic inflammation. We investigated the effects of 7-nitroindazole (7-NI), selective neuronal nitric oxide synthase (NOS) inhibitor, to oxidative status (OS) of brain capillaries. Adult Wistar rats were randomized at groups: control group (CG) (sham operated), sepsis group (GS) (cecal ligation and perforation with inoculation of Escherichia coli (ATCC 25922), 7-NI group (G7-NI), (30 mg/kg b/w i. p.) and 7-NI + sepsis group (G7-NIS), (7-NI was applied 30 minutes before operation). Lipid peroxidation index (LPI), nitrite concentration, superoxide dismutase (SOD) activity and superoxide anion (O2·-) content were determined 3, 6, 24 and 48 hour in each group. Cerebral capillaries were separated from non-vascular brain tissue using sucrose gradient. Compared to controls, LPI, nitrite and O2·- increased at SG. In the G7-NIS, LPI reached control values at the 24th and 48th hour, while nitrite were decreased at the 3 rd and 24 th hour, compared to controls. In the same group, O2·- decreased at the 3 rd, 6 th and 24 th hour, although SOD showed variable activity. The systematic nNOS inhibition with 7-NI forces OS on early terms of sepsis, but lately it contributes to the normalization of OS in cerebral capillaries.

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