Sequence Variants of Potato Virus Y in Field Grown Tobacco with Different Resistance Background Including Transgenic Ones

Palkovics, L. and Kryldakov, R. and Novák Nádudvari, Júlia and Józsa, Rita and Balázs, E. (2004) Sequence Variants of Potato Virus Y in Field Grown Tobacco with Different Resistance Background Including Transgenic Ones. Acta Phytopathologica et Entomologica Hungarica, 39 (4). pp. 315-324. ISSN 0238-1249

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Abstract

Experimental fields of a tobacco breeding station in Hungary at Debrecen were surveyed for potato virus Y (PVY) populations for over a decade. In this field, hundreds of crosses were grown regularly and most of them were lines and hybrids genetically resistant against potato virus Y. From 1993, transgenic tobacco lines bearing the coat protein gene of the new tuber necrotic strain of potato virus Y (NTN) were planted in this experimental field and tested yearly for their virus resistance and the durability of their resistance. During this period, no peculiar strain of PVY was observed, judging from symptoms or using biological tests. In the last five year period the biological observations of PVY isolates were combined with partial molecular analysis. The coat protein gene of PVY from all different isolates collected yearly was cloned, and the most variable 5' ends of the genes were sequenced and compared. All isolates belonged to the new tuber necrotic strain except one, showing the relative stability of the potato virus Y population in an area where genetically different tobacco lines, including transgenic ones, are grown and exposed to natural infection.

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