Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars

Kumar, S. and Srivastava, A. and Raj, S. K. (2005) Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars. Acta Phytopathologica et Entomologica Hungarica, 40 (3-4). pp. 205-211. ISSN 0238-1249

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Abstract

Molecular detection of Cucumber mosaic virus in various chrysanthemum cultivars was attempted by RT-PCR and Southern hybridization tests using CMV specific primers and cloned probes, respectively. A pair of primers was designed from a conserved region of the Cucumber mosaic virus coat protein (CMV-CP) gene, capable of amplifying a product of ~650 bp from various CMV strains. RT-PCR using the total nucleic acid from infected leaf samples and the specific primers resulted in positive amplification of an expected size band of ~650 bp in most of the samples. The identity of the PCR amplicons was checked by Southern hybridization using the a32P-labelled DNA probes prepared from the cloned coat protein gene of a well-identified strain of CMV isolated from Amaranthus. Positive signal of hybridization of PCR products and CMV probes confirmed the identity of fected chrysanthemum samples.

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