Towards the map based cloning of a recessive Fusarium resistance determinant from diploid Medicago sativa

Balogh, Márta and Miró, Krisztina and Dalmadi, Ágnes and Deák, Gábor and Petrovics, Tünde and Dudás, Brigitta and Kiss, Péter and Jakab, Júlia and Kiss, György Botond (2007) Towards the map based cloning of a recessive Fusarium resistance determinant from diploid Medicago sativa. Acta Phytopathologica et Entomologica Hungarica, 42 (2). pp. 279-289. ISSN 0238-1249

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Abstract

A monogenic recessive Fusarium resistance determinant was identified in a diploid Medicago sativa population. The Fusarium susceptible and resistant phenotypes of the individuals in an F2 segregation population was inferred from a biological test using injured root test. The resulting phenotypes were used to position the Fusarium resistance determinant on linkage group 6 of the Medicago sativa genetic map. Using the DNA based molecular markers genome walk was initiated from both sides of the genetic region constructing two contigs which are about 0.5 centiMorgan (∼500 kilobasepairs) apart. The introduction of this genetic determinant into tetraploid alfalfa cultivars was unsuccessful since only triploid and pentaploid hybrids could be recovered from the 4×-2× crosses. The isolation of the gene and establishment of the function of this recessive Fusarium determinant is under progress.

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