Triggering of a plant molecular defense mechanism: Increase in gene expression levels of transgene gsh I and poplar gene gsh 1 ( Populus × canescens ) by response to the DNA demethylating drug DHAC — an qRT-PCR analysis

Bittsánszky, A. and Gyulai, G. and Malone, R. P. and Gullner, G. and Kiss, J. and Czakó, M. and Márton, L. and Heszky, L. and Kőmíves, T. (2007) Triggering of a plant molecular defense mechanism: Increase in gene expression levels of transgene gsh I and poplar gene gsh 1 ( Populus × canescens ) by response to the DNA demethylating drug DHAC — an qRT-PCR analysis. Acta Phytopathologica et Entomologica Hungarica, 42 (2). pp. 235-243. ISSN 0238-1249

Text aphyt.42.2007.2.6.pdf Restricted to Repository staff only until 31 December 2027. Download (114kB)

Abstract

As DNA methylation patterns are inherited (‘epigenetic memory’) gshI transgenic poplar (Populus×canescens) clones (11 ggs and 6 Lgl ) were treated with the DNA demethylating drug DHAC (5,6-dihydro-5′-azacytidine hydrochloride) at 10−4 M for 7 days in order to study acquired plant molecular defense mechanisms in novel plant sources. In this study, the response of relative gene expression levels of transgene gsh I and poplar gene gsh 1 to DHAC treatment were analyzed by qRT-PCR ( q uantitative r everse t ranscriptase PCR). High expression levels of transgene gsh I were observed in the 6 Lgl clone (13.5-fold increase) compared to 11 ggs (1.0) sample. The expression level doubled (1.8-fold increase) in the DHAC-treated 6 Lgl samples but not in the 11 ggs clone (0.4-fold). Contrary to this, the relative copy number of transgene gsh I in the 6 Lgl clone was found to be 60% less (1.0) than in the 11 ggs sample (1.6). Relative expression level of endogenous poplar gene gsh 1 showed significantly higher responsiveness to DHAC-induced demethylation than the transgene gsh I with the highest expression level in the untransformed WT poplar (19.7-fold increase) compared to transformed clones of 6 Lgl (8.7-fold increase) and 11 ggs (2.5-fold increase), respectively. Competition in the reactivation capacity between transgene gsh I and poplar gsh 1 of 6 Lgl clone was also observed as the relative gene expression level of transgene gsh I increased from a high relative expression level (13.5) up by about twofold (1.8 times) rate (to 23.7) compared to poplar gsh 1 gene that increased by an 8.7 increment from a lower level (1.6 rel. expression) to 13.9.

Actions (login required)

Edit Item