Histopathology of spot blotch disease of wheat caused by Bipolaris sorokiniana (Teleomorph: Cochliobolus sativus )

Aggarwal, R. and Das, S. and Jahani, M. and Singh, D. V. (2008) Histopathology of spot blotch disease of wheat caused by Bipolaris sorokiniana (Teleomorph: Cochliobolus sativus ). Acta Phytopathologica et Entomologica Hungarica, 43 (1). pp. 23-30. ISSN 0238-1249

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Abstract

Bipolaris sorokiniana (Sacc.) Shoemaker (Teleomorph: Cochliobolus sativus (Ito and Kuribayashi) Drechs. Ex. Dastur) is a serious necrotrophic pathogen of wheat and barley in several countries. In this paper we report on mechanism of resistance and histopathological changes that occur on pathogen attack in resistant and susceptible genotypes. Callose deposition was observed in the epidermal cell wall of resistant genotype, Pusa T3336 at 2DAI. Lignin deposition around mesophyll tissues was also strongly observed by Phloroglucinol-HCl test in Pusa T3336. Four days after inoculation (4DAI), the pathogen colonized the leaf tissues showing inter and intracellular hyphae in susceptible genotype, Agralocal. In resistant genotype, no colonization was observed. The host tissue in susceptible genotype showed degeneration away from the site of colonization and also showed loss of structural integrity thereby indicating the role of toxin in pathogenesis. Separation of plasmalemma from the cell wall was observed due to loss of electrolytes and degradation of chloroplast at 5 DAI in the susceptible genotypes was prominently seen through light microscopy.

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