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LOCALIZATION OF A CUTICULAR PROTEIN DURING THE POSTEMBRYONAL DEVELOPMENT OF MANDUCA SEXTA

Molnár, KINGA and Borhegyi, NOÉMI H. and Csikós, GY. and Sass, M. (2001) LOCALIZATION OF A CUTICULAR PROTEIN DURING THE POSTEMBRYONAL DEVELOPMENT OF MANDUCA SEXTA. Acta Biologica Hungarica, 52 (4). pp. 457-471. ISSN 0236-5383

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Abstract

The pattern of cuticle protein synthesis by the epidermis of insects changes during the last larval, pupal and adult development, leading to an alteration in cuticular stucture and feature. We have isolated a pro- tein that had an apparent molecular mass of 33.1 kD from larval cuticle of Manduca sexta. Synthesis, transport and accumulation of MsCP33.1 were followed during metamorphosis by immunoblots and immunocytochemical methods using the antibody developed against this protein. Our data prove that the presence of MsCP33.1 in the larval cuticle is general while its appearance in the pupal or adult integu- ment is restricted only in the cuticle of wings and apodemes. We established that the synthesis of 33.1 kD protein is negatively regulated by moulting hormone (20-hydroxyecdysone). Possible roles for this cuticular protein are discussed.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: xEndre xSarvay
Date Deposited: 30 Oct 2017 09:16
Last Modified: 31 Aug 2021 23:15
URI: http://real.mtak.hu/id/eprint/66540

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