QUANTITATIVE MICROVASCULAR CHANGES DURING AZASERINE-INITIATED PANCREATIC CARCINOGENESIS

Pálfia, Z. and Réz, G. and Nagy, KRISZTINA (2001) QUANTITATIVE MICROVASCULAR CHANGES DURING AZASERINE-INITIATED PANCREATIC CARCINOGENESIS. Acta Biologica Hungarica, 52 (4). pp. 403-409. ISSN 0236-5383

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Abstract

Although angiogenesis is considered to be indispensable for continuous tumour growth, only very few studies have been published performing microvessel quantification during tumour progression. We mea- sured the tumour vascularity in different stages of rat pancreatic carcinogenesis induced by azaserine and promoted by raw soya flour-containing pancreatotrophic diet. Besides the tumour samples taken at 6 (atypical acinar cell nodules), 15 (adenomas) and 20 (localised adenocarcinomas) months after carcino- gen initiation, we also investigated 3 control groups: tumour-bearing host tissue of azaserine-treated rats and normal tissue of untreated rats kept on standard or pancreatotrophic diet. In contrast with the usual microvessel counting on hot spots, we determined microvascular surface density (SV) and volume den- sity (VV) by electron microscopic morphometry. There was no significant difference in these respect between the control groups. At month 6 after the azaserine induction SV and VV showed slight, non- significant decrease as compared to the host control. Both values remained unchanged until the 15th month and increased significantly by the 20th month. These results may indicate comparable growth rate of tumour and new microvessels in the premalignant stages of carcinogenesis while a more intense angio- genesis than tumour growth afterwards.

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