Efficient transposon mutagenesis in a wide range of phytopathogenic gram-negative bacteria

Czelleng, A. and Bozsó, Z. and Ott, P. G. and Besenyei, E. and Varga, G. J. and Szatmári, A. and Szabó, E. and Zsiros, L. R. and Klement, Z. (2009) Efficient transposon mutagenesis in a wide range of phytopathogenic gram-negative bacteria. Acta Phytopathologica et Entomologica Hungarica, 44 (1). pp. 19-24. ISSN 0238-1249

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Abstract

Compared to the known method of conjugation the frequency of transposon mutagenesis following conjugation was enhanced 11-fold by two hours of pre-incubation of recipient Pseudomonas viridiflava 1 on conjugation media. The increased frequency was ƒ = 1.3 × 10−4 . In other species of Pseudomonas, Pectobacteira and Xanthomonas high rates of transposon mutants were similarly obtained; however, in these strains the increased frequency was less than 5-fold.

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